Generating vesicular stomatitis virus pseudotype bearing the severe acute respiratory syndrome coronavirus spike envelope glycoprotein for rapid and safe neutralization test or cell-entry assay.
Identifieur interne : 003E91 ( Main/Exploration ); précédent : 003E90; suivant : 003E92Generating vesicular stomatitis virus pseudotype bearing the severe acute respiratory syndrome coronavirus spike envelope glycoprotein for rapid and safe neutralization test or cell-entry assay.
Auteurs : Jinying Ge [République populaire de Chine] ; Zhiyun Wen ; Xijun Wang ; Sen Hu ; Yonggang Liu ; Xiangang Kong ; Hualan Chen ; Zhigao BuSource :
- Annals of the New York Academy of Sciences [ 0077-8923 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Effet cytopathogène viral, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Infections à Rhabdoviridae (immunologie), Infections à Rhabdoviridae (médecine vétérinaire), Organismes exempts d'organismes pathogènes spécifiques, Poulets, Protéines de l'enveloppe virale (immunologie), Sécurité, Tests de neutralisation (médecine vétérinaire), Tests de neutralisation (normes), Virus de la stomatite vésiculeuse de type Indiana (génétique), Virus de la stomatite vésiculeuse de type Indiana (immunologie), Virus du SRAS (immunologie).
- MESH :
- génétique : Virus de la stomatite vésiculeuse de type Indiana.
- immunologie : Glycoprotéines membranaires, Infections à Rhabdoviridae, Protéines de l'enveloppe virale, Virus de la stomatite vésiculeuse de type Indiana, Virus du SRAS.
- médecine vétérinaire : Infections à Rhabdoviridae, Tests de neutralisation.
- normes : Tests de neutralisation.
- Animaux, Cellules Vero, Effet cytopathogène viral, Glycoprotéine de spicule des coronavirus, Organismes exempts d'organismes pathogènes spécifiques, Poulets, Sécurité.
English descriptors
- KwdEn :
- Animals, Chickens, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Membrane Glycoproteins (immunology), Neutralization Tests (standards), Neutralization Tests (veterinary), Rhabdoviridae Infections (immunology), Rhabdoviridae Infections (veterinary), SARS Virus (immunology), Safety, Specific Pathogen-Free Organisms, Spike Glycoprotein, Coronavirus, Vero Cells, Vesicular stomatitis Indiana virus (genetics), Vesicular stomatitis Indiana virus (immunology), Viral Envelope Proteins (immunology).
- MESH :
- chemical , immunology : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Vesicular stomatitis Indiana virus.
- immunology : Rhabdoviridae Infections, SARS Virus, Vesicular stomatitis Indiana virus.
- standards : Neutralization Tests.
- veterinary : Neutralization Tests, Rhabdoviridae Infections.
- Animals, Chickens, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Safety, Specific Pathogen-Free Organisms, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Delta G*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS-CoV in trans. The neutralization and infection blocking tests showed that the VSV Delta G*SG and SARS-CoV reacted similarly to SARS-CoV specific antiserum, suggesting the VSVDelta G*SG can be a safe replacement of the live SARS-CoV for neutralization test and cell-entry assay.
DOI: 10.1196/annals.1373.030
PubMed: 17135519
Affiliations:
Links toward previous steps (curation, corpus...)
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Le document en format XML
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sortKey="Chen, Hualan" sort="Chen, Hualan" uniqKey="Chen H" first="Hualan" last="Chen">Hualan Chen</name></author><author><name sortKey="Bu, Zhigao" sort="Bu, Zhigao" uniqKey="Bu Z" first="Zhigao" last="Bu">Zhigao Bu</name></author></analytic><series><title level="j">Annals of the New York Academy of Sciences</title><idno type="ISSN">0077-8923</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral</term><term>Membrane Glycoproteins (immunology)</term><term>Neutralization Tests (standards)</term><term>Neutralization Tests (veterinary)</term><term>Rhabdoviridae Infections (immunology)</term><term>Rhabdoviridae Infections (veterinary)</term><term>SARS Virus (immunology)</term><term>Safety</term><term>Specific Pathogen-Free Organisms</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term><term>Vesicular stomatitis Indiana virus (genetics)</term><term>Vesicular stomatitis Indiana virus (immunology)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Effet cytopathogène viral</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (immunologie)</term><term>Infections à Rhabdoviridae (immunologie)</term><term>Infections à Rhabdoviridae (médecine vétérinaire)</term><term>Organismes exempts d'organismes pathogènes spécifiques</term><term>Poulets</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Sécurité</term><term>Tests de neutralisation (médecine vétérinaire)</term><term>Tests de neutralisation (normes)</term><term>Virus de la stomatite vésiculeuse de type Indiana (génétique)</term><term>Virus de la stomatite vésiculeuse de type Indiana 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spécifiques</term><term>Poulets</term><term>Sécurité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Delta G*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS-CoV in trans. The neutralization and infection blocking tests showed that the VSV Delta G*SG and SARS-CoV reacted similarly to SARS-CoV specific antiserum, suggesting the VSVDelta G*SG can be a safe replacement of the live SARS-CoV for neutralization test and cell-entry assay.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Bu, Zhigao" sort="Bu, Zhigao" uniqKey="Bu Z" first="Zhigao" last="Bu">Zhigao Bu</name><name sortKey="Chen, Hualan" sort="Chen, Hualan" uniqKey="Chen H" first="Hualan" last="Chen">Hualan Chen</name><name sortKey="Hu, Sen" sort="Hu, Sen" uniqKey="Hu S" first="Sen" last="Hu">Sen Hu</name><name sortKey="Kong, Xiangang" sort="Kong, Xiangang" uniqKey="Kong X" first="Xiangang" last="Kong">Xiangang Kong</name><name sortKey="Liu, Yonggang" sort="Liu, Yonggang" uniqKey="Liu Y" first="Yonggang" last="Liu">Yonggang Liu</name><name sortKey="Wang, Xijun" sort="Wang, Xijun" uniqKey="Wang X" first="Xijun" last="Wang">Xijun Wang</name><name sortKey="Wen, Zhiyun" sort="Wen, Zhiyun" uniqKey="Wen Z" first="Zhiyun" last="Wen">Zhiyun Wen</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Ge, Jinying" sort="Ge, Jinying" uniqKey="Ge J" first="Jinying" last="Ge">Jinying Ge</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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